答：You should make sure that you triturate the cells very well. If the cells tend to form clumps， use a pasteur pipet and pass the cells several times. Then count the cell using Trypan Blue. Plate 1-5*10^4 per well， depending on your desired density. Usually， only the 60 wells in the middle should be used for plating cells. Wells on the edge will have a decreased volume of media， so you should not add any cells in there， but you still need to put media or H2O inside those 36 wells.
